Journal: Frontiers in cell and developmental biology

Article Title: Absence of Desmin Results in Impaired Adaptive Response to Mechanical Overloading of Skeletal Muscle.

doi: 10.3389/fcell.2021.662133

Figure Lengend Snippet: FIGURE 2 | Transition toward oxidative fibers is not impaired in DesKO plantaris in response to OVL. Representative images (A–D) of plantaris section immunostained for MHC-2a (red), MHC-2b (blue) and perlecan (green) from both genotype (Ctr and DesKO) in basal condition or after 1 month of OVL. Percentage of MHC-2a positive fibers (E) or MHC-2b positive fibers (F). Percentage of the surface of muscle section demonstrating succinate dehydrogenase activity (G). Scale bar = 100 µm. Data are given as means ± SEM. DesKO, Desmin knock-out mice; Ctr, Control mice; OVL, mechanical overloading; MHC, myosin heavy chain; SDH, succinate dehydrogenase. ns: non-significant, *p < 0.05, **p< 0.01, ***p< 0.001.

Article Snippet: Briefly, the sections were incubated with primary antibodies against heparan sulfate proteoglycan (Perlecan) (1:400, rat monoclonal, Millipore), myosin heavy chain (MHC)-2a (1:50, mouse monoclonal, clone SC-71, Developmental Studies Hybridoma Bank, University of Iowa) or MHC-2b (1:5, mouse monoclonal, clone BF-F3, Developmental Studies Hybridoma Bank, University of Iowa).

Techniques: Activity Assay, Knock-Out, Control

Journal: Stem Cell Research & Therapy

Article Title: Glioma-associated mesenchymal stem cells-mediated PD-L1 expression is attenuated by Ad5-Ki67/IL-15 in GBM treatment

doi: 10.1186/s13287-022-02968-z

Figure Lengend Snippet: Identification of MSCs derived from human glioma tissues. A and B Adherent growth patterns and fibroblastic morphology of GA-MSCs cultured in MSC media (× 100, scale bars = 1000 µm). C Immunofluorescence showed that GA-MSCs expressed MSC specific markers CD44 and CD105 (× 400, scale bars = 500 µm)

Article Snippet: The cells were incubated with primary antibodies against CD44 and CD105 (1:100, Proteintech, China), then overnight at 4 °C.

Techniques: Derivative Assay, Cell Culture, Immunofluorescence

Journal: Discover oncology

Article Title: Preclinical efficacy of CBR-5884 against epithelial ovarian cancer cells by targeting the serine synthesis pathway.

doi: 10.1007/s12672-024-01013-0

Figure Lengend Snippet: Fig. 5 CBR-5884-impaired stemness of EOC cells and enhanced chemotherapy sensitivity. A SKOV3 and ID8 cells were treated with CBR- 5884 (50 μM) or dimethyl sulfoxide (DMSO; control) for 5 days, and tumorsphere formation ability was accessed by sphere formation assay. Representative images (left) and quantification data (right) are shown. B Cell cytometry was performed to detect CD44 expression in SKOV3 and ID8 cells after treatment with 30 μM CBR-5884 for 48 h. Representative images (left) and quantification data (right) are shown. C Quanti- tative polymerase chain reaction assay was conducted to measure the mRNA expression levels of stem-related genes in SKOV3 cells, includ- ing ALDH1A1, NANOG, SOX2, EPCAM, and POU5F1 after treatment with 30 μM CBR-5884 for 48 h. D SKOV3 and ID8 cells were treated with DMSO, carboplatin (20 μM), CBR-5884 (30 μM), and carboplatin (20 μM) plus CBR-5884 (30 μM) for 72 h. Cell viability was measured using CCK-8 assay

Article Snippet: To detect CD44-positive cells, a FITC anti-mouse CD44 (IM7; 1:200 dilution, Cat. No. FITC-65117; Proteintech, USA) antibody was used.

Techniques: Control, Tube Formation Assay, Cytometry, Expressing, Polymerase Chain Reaction, CCK-8 Assay